Pharmacokinetics

More sensitive CAR-T positive cell detection

Flow Cytometry qPCR

Why do we detect CAR gene copy number?

Qualified copy number is one of the important quality control specifications of CAR-T drugs. On the one hand, it demenstrates there were T cells with CAR gene modification. On the other hand, since excessive CAR gene modification may lead to the risk of secondary oncogenesisit, it is also an indicator of safety. Copy number levels during clinical treatment can reflect the proliferation, distribution and survival of CAR-T cells. Accurate detection of CAR gene copy number is a key step in the quality control and clinical treatment of CAR-T drugs.

BioSwan's Advantages

sensitivity

Use well-designed primers and probes with high sensitivity to detect subtle copy number differences in clinical blood samples
specificity

Good specificity, no amplification curve was found in samples without target after 45 cycles
accuracy

Good accuracy, highly consistent with flow detection results
quality control

Have single copy internal reference gene, provide better quality control and absolute and relative quantification of CAR gene
anti-interference ability

The kit has strong anti-interference ability, wide sampling range, simple operation and is easy to use